Towards the Development of an Artificial Ovary

Ovarian preantral follicles often flatten and rupture during static culture in a tissue culture well. In this study, growth and morphology were evaluated for rat and mouse preantral follicles cultured for 72 hours in conventional culture wells and in suspension culture systems, consisting of orbiting test tubes and rotating-wall vessels. Follicles cultured in the orbiting test tubes had increased growth rates relative to the rate in the conventional culture wells, and experienced neither flattening nor rupture. The majority of follicles cultured in the rotating-wall vessel appeared to experience shear damage. In order to provide a potential barrier from shear stress during suspension culture, follicles were encapsulated in calcium alginate gels and growth and morphology were evaluated in the conventional and suspension culture systems. Encapsulated follicles more closely resembled an in vivo morphology and did not flatten nor rupture. Our studies suggest that suspension culturing in a rotating-wall vessel in combination with microencapsulation supports more natural three-dimensional follicular growth and morphology. The approach described herein is the first step toward the development of an artificial ovary. Additionally, this new culture system maintains follicles in a more natural morphology and will provide an important new avenue for further detailed investigation of the complex regulation of ovarian follicle development

Offshore wind farm layouts designer software's

Offshore wind energy can be considered one of the renewable energy sources with high force potential installed in marine areas. Consequently, the best wind farm layouts identified for constructing combined offshore renewable energy farms are crucial. To this aim, offshore wind potential analysis is essential to highlight the best offshore wind layouts for farm installation and development. Furthermore, the offshore wind farm layouts must be designed and developed based on the offshore wind accurate assessment to identify previously untapped marine regions. In this case, the wind speed distribution and correlation, wind direction, gust speed and gust direction for three sites have been analyzed, and then two offshore wind farm layout scenarios have been designed and analyzed based on two offshore wind turbine types in the Northwest Persian Gulf. In this case, offshore wind farm layouts software and tools have been reviewed as ubiquitous software tools. The results show Beacon M28 and Sea Island buoys location that the highest correlation between wind and gust speeds is between 87% and 98% in Beacon M28 and Sea Island Buoy, respectively. Considerably, the correlation between wind direction and wind speed is negligible. The Maximum likelihood algorithm, the WAsP algorithm, and the Least Squares algorithm have been used to analyze the wind energy potential in offshore buoy locations of the Northwest Persian Gulf. In addition, the wind energy generation potential has been evaluated in different case studies. For example, the Umm Al-Maradim buoy area has excellent potential for offshore wind energy generation based on the Maximum likelihood algorithm, WAsP algorithm, and Least Squares algorithm

Methodology framework for prioritisation of renewable energy sources in port areas

Ports play a crucial role in increasing the decarbonisation of urban environments to mitigate the environmental impacts of maritime transport and promote sustainable intermodal mobility. Various efforts have been made to increase energy self-sufficiency using renewable energy sources (RESs) in different ports worldwide. However, the ports played an essential role in the pollution process of the nearest cities due to the short distance and merging with urban areas. In this case, solar and wind were measured using the Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2) data of four Lazio province ports. Each RES was evaluated using 10 years of monthly data for mapping and 1 year of hourly data for potential assessment and energy converters installation. Furthermore, the time series method has been considered to design and develop better management of RESs for decision making monitoring the energy needs of ports. This time series method has been applied to the generated energy source based on various parameters of the RESs used in port

A parametric study of wave energy converter layouts in real wave models

Ocean wave energy is a broadly accessible renewable energy source; however, it is not fully developed. Further studies on wave energy converter (WEC) technologies are required in order to achieve more commercial developments. In this study, four CETO6 spherical WEC arrangements have been investigated, in which a fully submerged spherical converter is modelled. The numerical model is applied using linear potential theory, frequency-domain analysis, and irregular wave scenario. We investigate a parametric study of the distance influence between WECs and the effect of rotation regarding significant wave direction in each arrangement compared to the pre-defined layout. Moreover, we perform a numerical landscape analysis using a grid search technique to validate the best-found power output of the layout in real wave models of four locations on the southern Australian coast. The results specify the prominent role of the distance between WECs, along with the relative angle of the layout to dominant wave direction, in harnessing more power from the waves. Furthermore, it is observed that a rise in the number of WECs contributed to an increase in the optimum distance between converters. Consequently, the maximum exploited power from each buoy array has been found, indicating the optimum values of the distance between buoys in different real wave scenarios and the relative angle of the designed layout with respect to the dominant in-site wave direction

A primary offshore wind farm site assessment using reanalysis data: a case study for Samothraki island

The correct strategy for monitoring and assessing marine Renewable Energy Sources (RESs) is of great importance for local/national sustainable development. To achieve this goal, it is necessary to measure in the most precise possible manner the local/regional RESs potential. This is especially true for Offshore Wind (OW) energy potential, since the most precise techniques are long and expensive, and are not able to assess the RESs potential of large areas. Today, Remote Sensing (RS) satellites can be considered the most important land and marine observation tools. The RS tools can be used to identify the interested areas for future OW energy converters installations in large and small-scale areas. In this study, the OW energy potential has been analysed by means of a 40 years wind speed data from the European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis dataset of the Samothraki island surrounding area in the Mediterranean Sea. The OW speed potential has been analysed by means of monthly data from ECMWF Interim reanalysis (ERA-Interim) datasets using the Network Common Data Form (NetCDF) format. Automatically, analyses have been carried out using the Region Of Interest (ROI) tool and Geographical Information System (GIS) software in order to extract information about the OW speed assessment of the Samothraki island area. The primary results of this study show that the southwest area of Samothraki island has good potential for future OW farms installation (bottom fixed and floating version) in near and offshore areas. This study shows the OW energy potential per location, as well as the trend of OW speed, which has changed over the past 40 years in the Mediterranean Sea

Design optimization of ocean renewable energy converter using a combined Bi-level metaheuristic approach

In recent years, there has been an increasing interest in renewable energies in view of the fact that fossil fuels are the leading cause of catastrophic environmental consequences. Ocean wave energy is a renewable energy source that is particularly prevalent in coastal areas. Since many countries have tremendous potential to extract this type of energy, a number of researchers have sought to determine certain effective factors on wave converters’ performance, with a primary emphasis on ambient factors. In this study, we used metaheuristic optimization methods to investigate the effects of geometric factors on the performance of an Oscillating Surge Wave Energy Converter (OSWEC), in addition to the effects of hydrodynamic parameters. To do so, we used CATIA software to model different geometries which were then inserted into a numerical model developed in Flow3D software. A Ribed-surface design of the converter's flap is also introduced in this study to maximize wave-converter interaction. Besides, a Bi-level Hill Climbing Multi-Verse Optimization (HCMVO) method was also developed for this application. The results showed that the converter performs better with greater wave heights, flap freeboard heights, and shorter wave periods. Additionally, the added ribs led to more wave-converter interaction and better performance, while the distance between the flap and flume bed negatively impacted the performance. Finally, tracking the changes in the five-dimensional objective function revealed the optimum value for each parameter in all scenarios. This is achieved by the newly developed optimization algorithm, which is much faster than other existing cutting-edge metaheuristic approaches

Age shall not weary us: Deleterious effects of self-regulation depletion are specific to younger adults

Self-regulation depletion (SRD), or ego-depletion, refers to decrements in self-regulation performance immediately following a different self-regulation-demanding activity. There are now over a hundred studies reporting SRD across a broad range of tasks and conditions. However, most studies have used young student samples. Because prefrontal brain regions thought to subserve self-regulation do not fully mature until 25 years of age, it is possible that SRD effects are confined to younger populations and are attenuated or disappear in older samples. We investigated this using the Stroop color task as an SRD induction and an autobiographical memory task as the outcome measure. We found that younger participants (<25 years) were susceptible to depletion effects, but found no support for such effects in an older group (40–65 years). This suggests that the widely-reported phenomenon of SRD has important developmental boundary conditions casting doubt on claims that it represents a general feature of human cognition

An effective hyper-parameter can increase the prediction accuracy in a single-step genetic evaluation

The H-matrix best linear unbiased prediction (HBLUP) method has been widely used in livestock breeding programs. It can integrate all information, including pedigree, genotypes, and phenotypes on both genotyped and non-genotyped individuals into one single evaluation that can provide reliable predictions of breeding values. The existing HBLUP method requires hyper-parameters that should be adequately optimised as otherwise the genomic prediction accuracy may decrease. In this study, we assess the performance of HBLUP using various hyper-parameters such as blending, tuning, and scale factor in simulated and real data on Hanwoo cattle. In both simulated and cattle data, we show that blending is not necessary, indicating that the prediction accuracy decreases when using a blending hyper-parameter <1. The tuning process (adjusting genomic relationships accounting for base allele frequencies) improves prediction accuracy in the simulated data, confirming previous studies, although the improvement is not statistically significant in the Hanwoo cattle data. We also demonstrate that a scale factor, α, which determines the relationship between allele frequency and per-allele effect size, can improve the HBLUP accuracy in both simulated and real data. Our findings suggest that an optimal scale factor should be considered to increase prediction accuracy, in addition to blending and tuning processes, when using HBLUP.Mehdi Neshat, Soohyun Lee, Md. Moksedul Momin, Buu Truong, Julius H. J. van der Werf, and S. Hong Le